Genetic analysis of a white double-flowering Nicotiana alata is being investigated. Self-pollination of the double-flowering plant produced all double progeny. Reciprocal hybridization of the double-flowered selection with N. alata cultivars produced nondouble F1 progeny that segregated 3:1 (nondouble to double) in the F2 generation. Reciprocal backcrosses of F1 plants to the parents resulted in nondouble progeny when backcrossed to the nondouble parent and 1:1 segregation when backcrossed to the double parent. Intercross of F1 plants resulted in progeny segregating 3:1. Double flowering habit has been transferred to white, red, salmon, green, and bicolor N. alata. Results suggest double flowering is under nuclear control regulated by a recessive allele.