RAPD markers were used to determine the level of heterozygosity transmitted via 2n gametes from V. darrowi cv. Florida 4b (Fla 4B) to interspecific hybrids with tetraploid V. corymbosum cv. Bluecrop. The tetraploid hybrid US 75 was found to contain 70.6% of Fla 4B's heterozygosity, a value consistent with a first division restitution (FDR) mode of 2n gamete production. Crossovers during 2n gamete formation were evidenced by the absence of 16 dominant alleles of Fla 4B in US 75, and direct tests of segregation in a diploid population involving Fla 4B. RAPD markers that were present in both Fla 4B and US 75 were used to determine the mode of inheritance in a segregating population of US 75 × V. corymbosum cv. Bluetta. More than 30 homozygous pairs of alleles were located that segregated in a 5:1 ratio, indicating US 75 undergoes tetrasomic inheritance.
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