The Application of Genetic Diagnostics to Plant Genome Analysis and Plant Breeding

in HortScience
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  • 1 1Agricultural Products, E.I. Dupont de Nemours, P.O. Box 80402, Wilmington, DE 19880-0402
  • | 2 2Universita Di Udine, Dip Di ProduzioneVegetale, Via Delle Scienze 208, Udine, I-33100, Italy
  • | 3 3Scottish Crop Research Ins., Invergowrie, Dundee, DD2 5DA Scotland, United Kingdom.

DNA-based diagnostics are now well-established as a means to assay diversity at the locus, chromosome, and whole-genome levels. As technology has advanced, DNA sequence-based assays have become easier to use, more efficient at screening for nucleotide sequence-based polymorphisms, and available to a wider cross-section of the research community. A review of the use of molecular markers in several different areas of genetics and plant breeding will be presented, as well as a discussion about their advantages and limitations. Recent advances in several areas of technology development and laboratory automation will also be presented, including a summary of direct comparison of different DNA marker systems against a common set of soybean cultivars.

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