Seventeen strawberry (Fragaria ×ananassa Duchesne) cultivars and six selections were tested under greenhouse conditions for susceptibility to leaf spot induced by Mycosphaerella fragariae (Tul.) Lindau. The level of susceptibility was evaluated based on maximum disease severity and area under the disease progress curve (AUDPC). The 23 genotypes were ranked based on AUDPC and grouped according to their susceptibility. Cluster analysis for AUDPC gave four groups corresponding to low, moderate, high, and very high susceptibility to leaf spot. `Annapolis', `Chambly', `Glooscap', `Redcoat', and `Veestar' consistently showed a low level of susceptibility. The selections SJ89700-1 and SJ8518-11 and `Tribute' showed a very high level of susceptibility, and the remaining cultivars were grouped as either moderately or highly susceptible.