DNA Fingerprints in Plants Using Simple-sequence Repeat and Minisatellite Probes

in HortScience
View More View Less
  • 1 Department of Fruit Breeding and Genetics, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
  • 2 Department of Genetics, The Faculty of Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
  • 3 Molekulare Humangenetik, MA, Ruhr–Universität, Universitätsstrasse 150, W-4630 Bochum, Germany

Plant genomes contain polymorphic repetitive sequences that can be used as DNA markers. Minisatellites (16 to 64 bp per repeat) and simple-sequence repeats (2 to 6 bp per repeat) are the most polymorphic markers found in plant and animal genomes. In this study, the hybridizations between genomic DNA and variable number of tandem repeat probes were examined in Arabidopsis thaliana L. (Heynn), onion (Allium cepa L.), tomato (Lycopersicon esculentum L.), wheat (Triticum aestivum L.), avocado (Persea americana Mill.), litchi (Chinensis Sonn.), mango (Mangifera indica L.), and Carica species. Some of the probes detected polymorphic sequences in all the species, but others were useful only for one or two species. None of the probes gave clear band patterns in either onion or wheat. The in-gel hybridization method was similar to Southern blot hybridization using the simple-sequence repeat probes.

If the inline PDF is not rendering correctly, you can download the PDF file here.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 230 39 7
PDF Downloads 158 64 7