SIMULTANEOUS SCREENING FOR VIRUS AND NEMATODE RESISTANCE IN SOUTHERNPEA, VIGNA UNGUICULATA

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  • 1 Department of Horticulture, 101 Funchess Hall, Auburn University AL 36849

Screening for resistance to blackeye cowpea mosaic virus (BlCMV) and rootknot nematode on the same plant is possible if the two pathogens do not interact significantly. To determine if such interactions were present four cultivars were planted in 72-cell styrofoam flats, with a combination of BlCMV and nematode inoculations (--, -+, +-, and ++). `Freezegreen' is known to be susceptible to both pathogens, `Mississippi Silver' is resistant to both, `California Blackeye #5' is susceptible to BlCMV, and `Worthmore' is resistant to BlCMV. Nematode treated seeds were inoculated at planting with 2,000 eggs of (Meloidogyne incognita Race 3); BlCMV was inoculated on primary leaves a week later. Plants were visually rated for symptoms: either negative or positive for BlCMV and 1-5, no galls and heavily galled respectively, for rootknot. Analyses of variance using percentage of plants negative for virus symptoms or average nematode score as the dependent variable, resulted in non-significant virus × nematode interactions. Results by cultivar indicated simultaneous screening did not change their resistance/susceptible classifications.

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