RAPD BANDING PATTERNS IN SWEETPOTATO INFECTED WITH FUSARIUM LATERITIUM

in HortScience
View More View Less
  • 1 Department of Horticulture, Louisiana State University Agricultural Center, Baton Rouge, LA 70803

Chlorotic Leaf Distortion (CLD) is a common disease of sweetpotato caused by Fusarium lateritium. This fungus is unique among Fusarium species in that it grows on the epidermis of leaves and shoot tips of sweetpotato. Fusarium lateritium appears as a white epiphytic material and under bright sunlight causes leaf chlorosis. When cloudy weather persists for several days, all symptoms disappear.

Researchers who use RAPD to examine banding patterns of sweetpotato DNA assume that foreign DNA present in the cTAB extract is quantitatively low and will not appreciably amplify and appear as bands. In this study we found the modified cTAB procedure used to amplify sweetpotato DNA also amplifies DNA of Fusarium lateritium cultures. DNA banding patterns of infected leaves was compared with those free of the disease. No differences in banding patterns were observed in this preliminary study.

If the inline PDF is not rendering correctly, you can download the PDF file here.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 34 12 0
PDF Downloads 37 11 0