Screening Tomato Seedlings for Resistance to Bacterial Spot

in HortScience
Authors:
G. Cameron SomodiUniversity of Florida, Gulf Coast Research and Education Center, 5007 60th Street East, Bradenton, FL 34203

Search for other papers by G. Cameron Somodi in
ASHS
Google Scholar
PubMed
Close
,
J.B. JonesUniversity of Florida, Gulf Coast Research and Education Center, 5007 60th Street East, Bradenton, FL 34203

Search for other papers by J.B. Jones in
ASHS
Google Scholar
PubMed
Close
,
J.W. ScottUniversity of Florida, Gulf Coast Research and Education Center, 5007 60th Street East, Bradenton, FL 34203

Search for other papers by J.W. Scott in
ASHS
Google Scholar
PubMed
Close
, and
J.P. JonesUniversity of Florida, Gulf Coast Research and Education Center, 5007 60th Street East, Bradenton, FL 34203

Search for other papers by J.P. Jones in
ASHS
Google Scholar
PubMed
Close

A `spray-inoculation seedling screening procedure was developed for detecting resistance to Xanthomonas campestris pv. vesicatoria (Doidge) Dye, causal agent of bacterial spot of tomato (Lycopersicon esculentum Mill.). Two-week-old transplants were preconditioned under 95% humidity for 16 hours before spray inoculation and then rated for bacterial spot 2 weeks later. Resistant plants could also be distinguished from susceptible genotypes using a modified bacterial speck [Pseudomonas syringae pv. tomato (Okabe) Young, Dye, and Wilkie] screening procedure (cotyledon-dip technique). When results of both screening methods were compared to field ratings from three previous seasons, significant correlations were more frequently observed for the spray-inoculation method. In Summer 1991, individual plants were evaluated by the spray-inoculation technique and then were placed in the field to determine susceptibility under field conditions. Correlations (r = 0.28 to 0.34) between spray-inoculation seedling screening ratings and field ratings, although low, were significant (P ≤ 0.0001). More than 90% of susceptible plants could be eliminated, saving labor, space, and time.

  • Collapse
  • Expand