1010 DELAYED PETAL SENESCENCE IN TRANSGENIC CARNATION USING ANTISENSE ACC OXIDASE

in HortScience
Authors:
Keith W. SavinCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Stanley C. BaudinetteCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Michael W. GrahamCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Ellen L-J. WhiteCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Michael Z. MichaelCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Ann BaylyCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Chin-Yi LuCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Stephen F. ChandlerCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Edwina C. CornishCalgene Pacific Pty Ltd. 16 Gipps St, Collingwood, Australia, 3066

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Ethylene is essential for the senescence process in many fruit and flowers. In the last two steps in the biosynthesis of ethylene in plants ACC synthase converts S-adenosyl methionine to 1-aminocyclopropane-1-carboxylic acid(ACC). ACC oxidase (ACO) then degrades ACC to ethylene. Inhibitors of ethylene synthesis, such as amino-oxyacetic acid, and of the response to ethylene, such as silver thiosulphate, delay or prevent senescence. By expression of an antisense version of ACO RNA, we have generated two varieties of transgenic carnation which produce flowers with an extended vase life. These were produced using the cultivars Red Sim and White Sim. Flowers from these plants produce very little ethylene and normally fail to display the inrolling phenotype typical of senescence in this species. At the time after harvest when inrolling would normally lake place (5 days), the antisense ACO flowers produce only barely detectable levels of endogenous ACO mRNA or ACS (ACC Synthase) mRNA. Exposure to exogenous ethylene(100ppm) induces inrolling and production of ACS and ACO mRNA species. Such carnations will be valuable both as a commercial product and as a tool for further exploring the role of ethylene in carnation flower senescence and leaf wound response.

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