894 PB 502 COMPARISON OF TWO CULTURE VESSEL ENCLOSURES ON THE GROWTH OF POTATO IN VITRO

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  • 1 The Bionetics Corporation (ncy, clm, bvp) and NASA Biomedical Operations Office (rmw), Kennedy Space Center, FL 32899

In vitro growth of white potato (Solanum tuberosum L.) cv. Norland was investigated comparing two types of culture vessel enclosures. Nodal cuttings were aseptically transferred to 25 × 150 mm glass culture vessels containing a solidified medium consisting of Murashige and Skoog salts, 1% sucrose, and pH adjusted to 5.8. The vessels were capped with loose-fitted (1 cm gap between the top of the vessel and the top of the cap) Magenta 2-way caps or Bellco Kap-uts with calculated air changes hr-1 of 2.25 and 1.43, respectively. Instantaneous PPF attenuations of 15% for Magenta caps and 23% for Bellco caps were also measured. The cultures were maintained for 28 d in an environmental growth chamber under Daylight fluorescent lamps with a 16 hr light/8 hr dark photoperiod, 200 μmol m-2s-1 PPF maintained for each cap type, constant 23 C, 65% relative humidity, and CO2 enrichment of 1000 μmol mol-1 external to the culture vessels. Results showed that increased plantlet height, fresh weight, and dry weight was obtained for plantlets cultured with Magenta caps. The differences in growth and internal CO2 concentration of the vessels correlated well with the difference in air exchange rates, suggesting that increased air exchange of culture vessels resulted in increased mixotrophic plantlet growth.

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