890 PB 472 SOMACLONAL VARIATION IN PINEAPPLES

in HortScience

The objective of this project is to propagate disease-free clones of fresh market pineapples for growers. To expedite the distribution process, sterile cultures were obtained from the USDA/ARS Germplasm Repository, Hilo, Hawaii of pineapple clones 119, 155 and 159. Plantlets were placed on modified Murashige and Skoog (MS) medium with one half of the salts and 2 uM BAP and 1 uM IAA. The cultures were transferred to modified MS medium with one half of the salts, no growth regulators, and solidified with Phytagel. Plantlets were transplanted at one-inch size into peatlite mix. At 4-6 inch size, the plants were transplanted in growers fields. Early observations indicate difference between clones in kinds and frequency of “off-types.” Compared with surrounding plants, “off-types” were categorized as being dwarfed, variegated, thorny leaved, and pigmentation modified.

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