884 PB 302 PLANT REGENERATION FROM OVARY TISSUES OF EASTER LILY

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  • 1 Dept. of Botany, North Dakota State University, Fargo, ND 58105
  • | 2 Dept. of Crop and Weed Sciences, North Dakota State University, Fargo, ND 58105

Influences of culture media, sucrose, and growth regulator concentrations on plant regeneration from Easter lily (Lilium longiflorum L.) were investigated. Ovary tissues excised from unopened flower buds (3-10 cm long) were cultured on either B-5 medium or MS medium containing 2, 5, or 10% sucrose, 0.8% agar or Phytagel, and varying concentrations of 2,4-D, kinetin, naphthaleneacetic acid (NAA) and benzyladenine (BA). Callus formation from explants was more prolific on MS medium than on B-5 medium and when cultures were initially placed in the dark for 20 days. Cultures grew best when the medium contained 5% sucrose. Shoot differentiation from callus was maximum when MS medium contained 1 mg/liter 2,4-D and 2 mg/liter BA. Roots developed when shoots were placed on the same medium with 1 mg/liter 2,4-D, 0.1 mg/liter NAA and 0.1 mg/liter kinetin. Rooted plants were successfully transferred into soil medium in a greenhouse.

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