Influences of culture media, sucrose, and growth regulator concentrations on plant regeneration from Easter lily (Lilium longiflorum L.) were investigated. Ovary tissues excised from unopened flower buds (3-10 cm long) were cultured on either B-5 medium or MS medium containing 2, 5, or 10% sucrose, 0.8% agar or Phytagel, and varying concentrations of 2,4-D, kinetin, naphthaleneacetic acid (NAA) and benzyladenine (BA). Callus formation from explants was more prolific on MS medium than on B-5 medium and when cultures were initially placed in the dark for 20 days. Cultures grew best when the medium contained 5% sucrose. Shoot differentiation from callus was maximum when MS medium contained 1 mg/liter 2,4-D and 2 mg/liter BA. Roots developed when shoots were placed on the same medium with 1 mg/liter 2,4-D, 0.1 mg/liter NAA and 0.1 mg/liter kinetin. Rooted plants were successfully transferred into soil medium in a greenhouse.