671 PB 145 GENETIC LINKAGE MAPPING IN PEACH

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  • 1 Department of Biological Sciences, Clemson University, Clemson, SC 29634
  • | 2 Appalachian Fruit Research Station, Agricultural Research Service, U.S. Department of Agriculture, Kearneysville, WV 25430
  • | 3 Department of Horticulture, Clemson University, Clemson, SC 29634
  • | 4 INRA Centres de Recherches de Bordeaux, 33883 Villenave D'Ornon Cedex, France

We have constructed a genetic linkage map of peach consisting of RFLP, RAPD, and morphological markers, based on 78 F2 individuals derived from the self-fertilization of four F1 individuals originating from a cross between `New Jersey Pillar' and KV 77119. This progeny set was chosen because parental genotypes exhibit variation in canopy shape, fruit flesh color, and flower petal color, size, and number. The segregation of 81 markers comprised of RFLP, RAPD and morphological loci was analyzed. Low copy genomic and cDNA probes were used in the RFLP analysis. The current genetic map for the WV family contains 57 markers assigned to 9 linkage groups, which cover 520 cM of the peach nuclear genome. The average distance between two adjacent markers was 9 cM. Linkage was detected between Pillar (Pi) and double flowers (Dl). RFLP markers loosely linked to Pi, flesh color (Y), and white flower (W) loci were found. Twenty-four markers remain unassigned.

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