471 PB 361 GENETIC ENGINEERING OF STARCH USING CLONED STARCH BRANCHING ENZYME GENES

in HortScience

During plant starch biosynthesis, starch branching enzymes (SBE) catalyze a-1,6 branch point formation in starch, and thus are responsible for many properties of the starch polymer. Recently we have cloned cDNAs encoding the two major branching enzymes in developing maize endosperm, SBEI and SBEII. These genes are being used to alter starch biosynthesis via genetic engineering strategies. Transgenic tobacco plants with sense and antisense constructs of SBEI and SBEII have been produced. No major difference in the phenotypes of control and transgenic plants have been observed. Initial experiments demonstrated the transcription of the introduced genes. Enzyme levels and the molecular properties of the starch in the transgenic plants will be determined. These experiments will provide us with information as to the role of starch branching enzymes in starch biosynthesis, the feasibility of creating novel starch, and the effect altered starch has on plastid development and photosynthesis.

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