464 PB 321 IDENTIFICATION OF RAPD MARKERS ASSOCIATED WITH QTLs CONTROLLING DROUGHT TOLERANCE IN PINTO BEANS

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  • 1 Jorge Acosta G. Michigan State University, Crop and Soil Sciences Dept., East Lansing, MI 48824

Common beans, considered sensitive to moisture stress, are an important commodity in developing countries such as the Mexican Highlands where intermittent drought conditions are prevalent during the growing season. The selection and development of high performing cultivars under drought stress is confounded by the quantitative nature of drought tolerance. To employ indirect selection in earlier generations, RAPD markers were identified that associated with QTLs controlling performance under drought stress. RAPD markers are preferred for use in Phaseolus vulgaris, over RFLPs, because they generate polymorphisms between genetically related germplasm. 48% of 620 arbitrary primers screened against three parents of two F6 derived recombinant inbred pinto populations were polymorphic for one or more bands. These polymorphisms were screened against RILs in each population and associations were determined using one-way ANOVAs and Mapmaker. Yield data used for determination of associations was collected over five years in MI and Mexico where both stress and non stress treatments were applied.

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