463 PB 298 IDENTIFICATION OF RAPD MARKERS ASSOCIATED WITH CANNING QUALITY IN NAVY BEANS

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  • 1 Michigan State University, Crop and Soil Sciences Dept., East Lansing, MI 48824

Ninety-eight percent of the navy beans (Phaseolus vulgaris) grown in the US are processed. Thus, new cultivars considered for release must meet industry standards. Canning quality behaves as a classical QTL which precludes its selection and evaluation in early generations. Such delays add a measure of inefficiency to a breeding program. Indirect selection for canning quality using molecular markers could increase efficiency. RAPD markers are more useful than RFLP's, in Phaseolus, due to a simpler protocol and a higher level of polymorphism within genetically related cultivars. Three populations of RIL's, derived from crosses between cultivars with standard and sub-standard canning quality, were screened to identify markers associated with canning quality. Material for evaluation was grown at two locations, in three replications and processed, in the Food Science Processing Lab, following industry standards. Quality traits measured were: processed texture, color and appearance. Associations of putative markers with canning quality were identified using ANOVA and Mapmaker programs

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