250 INDUCTION AND AVOIDANCE OF VITRIFICATION OF MICROPROPAGATED ONION (ALLIUM CEPA L.)

in HortScience
View More View Less
  • 1 University of Illinois, Department of Horticulture, 1102 S. Goodwin Ave., Urbana, IL. 61801

The objective of this research was to induce vitrification in onion (Allium cepa L. cv. `White Ebeneezer'); then use this information to make suggestions on how to avoid vitrification of micropropagated plants. There were no differences in vitrification percentage when shoot tip explants were isolated, sterilized and placed on MS medium (8 g.L-1 agar) supplemented with 0.16 uM NAA and varying (0.0 to 70.0 uM) levels of BA. When agar was replaced by gelrite (MS medium with 4.4 uM BA and 0.16 uM NAA), vitrification increased when gelrite concentrations decreased from 2.0 to 1.0 g.L-1. More vitrification occurred when shoot tips were supported on a synthetic cosmetic puff in liquid medium or when agar was reduced to 4.0 g.L-1 than when supported on a cosmetic puff in 8 g.L-1 agar or on 8 g.L-1 agar alone.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 52 13 3
PDF Downloads 83 30 4