043 IMPROVING THE MICROPROPAGATION OF PISTACIA VERA L.: EFFECTS OF AUXIN, TEMPERATURE, AND METHYL JASMONATE

in HortScience
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  • 1 Departament de Genètica Vegetal, IRTA, Centre de Cabrils, 08348 Cabrils (Barcelona), Spain

Micropropagation of Pistacia vera L. `Mateur' was improved with the addition of methyl jasmonate (MeJA) to the multiplication and rooting media. Shoot tip cultures established from grafted trees were maintained on a modified Murashige and Skoog medium containing 5μM BA and 0.05μM IBA. Addition of 1μM MeJA improved the multiplication rate but inhibited shoot growth when present at higher concentrations. Rooting experiments comparing the effects of IAA, NAA, or IBA at 0, 1, 3.2, 10, or 31.6 μM demonstrated a significant effect of temperature on auxin root induction for shoots maintained at 25 or 28°C. At 25°C NAA was better than IAA or IBA, whereas no differences among auxins were observed at 28°C. Addition of MeJA (0, 0.3, 1, 3.2, or 10 μM) to the best rooting media significantly improved the rooting percentage and root number. Greater than 80% rooting was obtained when 1 μM MeJA was added to both the root induction medium, containing 31.6 μM NAA, and the auxin-free medium. In addition, transfer to soil and acclimation was easier for plantlets rooted in MeJA-containing medium.

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