025 MICROPROPAGATION OF HESPERALOE PARVIFLORA

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  • 1 Department of Plant Science, University of Arizona, Tucson, AZ 85721

Hesperaloe parviflora is a useful xeric landscape plant. Two methods of shoot culture initiation were developed. Shoots were initiated indirectly through the use of callus derived from pieces of young inflorescences. Callus was initiated on modified Murashige and Skoog medium with 4.52 μM 2,4-dichlorophenoxyacetic acid and 2.32 μM kinetin. Callus produced shoots when placed on modified MS medium with 6.0 μM zeatin riboside. Direct initiation of shoots was also accomplished using the bottom four floral buds of young inflorescences. Buds were placed on modified MS media containing either benzylaminopurine or kinetin at 0.1, 1.0, or 10.0 μM or zeatin riboside at 6.0 μM. The most shoots were produced by the medium containing 6.0 μM zeatin riboside. Preliminary results indicate optimum shoot production with 2.0 to 4.0 μM BA or 6.0 μM zeatin riboside.Hesperaloe parviflora micro-shoots were rooted on one quarter strength MS medium and ex vitro.

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