Introgressing Cold-tolerant Ogura Cytoplasm from Rapeseed into Pak Choi and Chinese Cabbage

in HortScience

Cold-tolerant, Ogura male-sterile, somatic hybrid rapeseed (Brassica napus L.) lines were used as maternal parents in two independent introgression experiments. In one experiment, an atrazine-sensitive B. napus (aacc) somatic hybrid was crossed directly with a male-fertile pak choi (B. campestris Chinensis Group, aa) accession. Allotriploid (sac) progeny were then backcrossed to the recurrent pak choi parent. Forty-five percent of the progeny from the first backcross were determined to be diploids (aa). In the other experiment, an atrazine-resistant B. napus somatic hybrid was crossed first to a bridge line. Three additional backcross generations to Chinese cabbage (B. campestris Pekinensis Group) resulted in Chinese cabbage resistant to black rot (Xanthomonas campestris pv. campestris). These materials may be useful for production of B. campestris hybrid vegetable seed.

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