A Simple Transformation System Using Adventitious Shoot Multiplication of Juneberry

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  • 1 Departments of Crop and Soil Sciences and Entomology, 202 Pesticide Research Center, Michigan State University, East Lansing, MI 48824-1311

A simple gene transfer method based on Agrobacterium -mediated transformation of adventitious multiplication of Juneberry (Amelanchier laevis L.) basal shoots is described. Evidence is presented for successful integration and expression of a transformed gene in greenhouse-grown transgenic plants. This method can transform woody perennials that are difficult to regenerate from leaf disks, protoplasts, or other tissue culture regimens.

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