Tissue Culture of Cowpea and Pigeonpea

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  • 1 Department of Agriculture, Lincoln University, Jefferson City, MO 65102-0029

Tissue culture of four cowpea (Vigna unguiculata) and two pigeonpea (Cajanus cajan) genotypes was tested on Blaydes' medium supplemented with different hormone concentrations. Explants of cotyledonary nodes, cotyledons and leaves of the cowpea genotype IT82E-16, IT64E-124, Pinkeye Purple Hull and MN13 produced callus after 4 weeks in Blaydes' medium. The hormone combinations in the medium were 2-l dichlorophenoxy acetic acid (2.4-D) (2 mg/liter) and kinetin at 0.05, 0.1, 0.5 mg/liter, or 2,4-D and thidiazuron (TDZ) at 2.2, 4.4 and 6.6 mg/liter or benzylaminopurine (BA) at 2.25, 4.5 or 6.75 mg/liter. Shoots occured on cotyledonary nodes of Pinkeye Purple Hull In the TDZ (6.6 mg/liter). Roots were produced from the leaf and cotyledonary nodes of Pinkeye Purple Hull and on cotyledons of IT-64E-124 cultured In media with kinetin (0.5 mg/liter). Leaf and cotyledon explants of pigeonpea genotype; ICPL 146 1965HK and ICPL 65024 produced callus and some shoots in BA (2.25 mg/liter) after 4 weeks. The callus when subcultured on BA (0.5 mg/liter) and NAA (0.1 mg/liter) produced shoots. Regenerated shoots rooted in the Blaydes' medium with kinetin (0.01 mg/liter) and NAA (0.6 mg/liter).

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