Characterization of Red Raspberry Cultivars and Selections Using Isoenzyme Analysis

in HortScience
Authors:
J.C. CousineauAgriculture Canada, Research Station, 6660 N. W. Marine Drive, Vancouver, B.C. V6T-1X2, Canada

Search for other papers by J.C. Cousineau in
ASHS
Google Scholar
PubMed
Close
,
A.K. AndersonAgriculture Canada, Research Station, 6660 N. W. Marine Drive, Vancouver, B.C. V6T-1X2, Canada

Search for other papers by A.K. Anderson in
ASHS
Google Scholar
PubMed
Close
,
H.A. DaubenyAgriculture Canada, Research Station, 6660 N. W. Marine Drive, Vancouver, B.C. V6T-1X2, Canada

Search for other papers by H.A. Daubeny in
ASHS
Google Scholar
PubMed
Close
, and
D.J. DonnellyDepartment of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, Macdonald Campus, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Que. H9X 3V9, Canada

Search for other papers by D.J. Donnelly in
ASHS
Google Scholar
PubMed
Close

Isoenzyme staining of horizontal starch gels was used to characterize 23 cultivars and three advanced selections of red raspberry (Rubus idaeus L.). The genotypes were separable using the enzymes malate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, and triose phosphate isomerase. In addition, staining for isocitrate dehydrogenase and shikimate dehydrogenase revealed polymorphisms in some cultivars. By combining these results with those obtained for 78 previously tested cultivars, 75 of the 104 (72%) genotypes tested were uniquely characterized using the six isoenzymes.

  • Collapse
  • Expand