Characterization of Red Raspberry Cultivars and Selections Using Isoenzyme Analysis

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  • 1 Agriculture Canada, Research Station, 6660 N. W. Marine Drive, Vancouver, B.C. V6T-1X2, Canada
  • | 2 Department of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, Macdonald Campus, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Que. H9X 3V9, Canada

Isoenzyme staining of horizontal starch gels was used to characterize 23 cultivars and three advanced selections of red raspberry (Rubus idaeus L.). The genotypes were separable using the enzymes malate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, and triose phosphate isomerase. In addition, staining for isocitrate dehydrogenase and shikimate dehydrogenase revealed polymorphisms in some cultivars. By combining these results with those obtained for 78 previously tested cultivars, 75 of the 104 (72%) genotypes tested were uniquely characterized using the six isoenzymes.

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