THE USE OF IN VITRO MIST CULTIVATION FOR THE PROPAGATION AND HARDENING-OFF OF VIRUS-FREE POTATO PLANTLETS.

in HortScience
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  • 1 Dept. of Horticulture Science, University of Saskatchewan, Saskatoon, SK S7N 0W0

The use of an in vitro mist culture systems for the growth of virus free shoots of several potato cultivars has been demonstrated using the “Mystifier®”, several different media and growth regulator concentrations have been used to monitor the efficiency of shoot multiplication and growth. Nodal cuttings of sterile in vitro virus free cultures were used as inocula and the mist was applied at various rates in order to assess optimum sucrose concentrations and mineral concentrations. Murashige and Skoog medium was used and diluted to half and quarter strength.

Hardening off the resulting shoots was achieved by passing HEPA filtered air at various rates over the plantlets still in the culture vessel. Conditions for high efficiency hardening off of cultured material has been defined such that plantlets can be transferred directly to greenhouse conditions.

The implications and opportunities that are indicated by this work are discussed.

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