IN VITRO PROPAGATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM SEEDLING EXPLANTS

in HortScience
Authors:
Yaseen Mohamed-YaseenUniv of Fla, Tropical Research and Educational Center, 18905 SW 280 St, Homestead, FL 33030

Search for other papers by Yaseen Mohamed-Yaseen in
ASHS
Google Scholar
PubMed
Close
,
Raymond J. SchnellUSDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Road, Miami, FL 33158

Search for other papers by Raymond J. Schnell in
ASHS
Google Scholar
PubMed
Close
,
Robert J. KnightUSDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Road, Miami, FL 33158

Search for other papers by Robert J. Knight in
ASHS
Google Scholar
PubMed
Close
, and
T. L. DavenportUniv of Fla, Tropical Research and Educational Center, 18905 SW 280 St, Homestead, FL 33030

Search for other papers by T. L. Davenport in
ASHS
Google Scholar
PubMed
Close

Guava (Psidium guajava L.) is an exceptional source of vitamin. C. It is also considered to be the most important cultivated species of the Myrtel family. Shoot tip and stem node were taken from seedling germinated in Murashige and Skoog medium (MS) and cultured in the same medium supplemented with 1-3mg/l benzylaminopurine (BA) and 0.1mg/l naphthaleneacetic acid (NAA) or 0.2-2mg/l thidiazuron (TDZ) and 0.1mg/l NAA. Multiple shoots (4-6) were obtained in 4-5 weeks from culture in 1-2mg/l BA and 0.1mg/l NAA, while TDZ caused abnormal shoot growth. Shoots were rooted successfully with 100% frequency in MS medium containing 2mg/l indolebutyric acid and further elongation of shoots was achieved in MS medium, supplemented with lg/l activated charcoal. Regenerated plantlets were successfully established in soil.

  • Collapse
  • Expand

 

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 16 16 10
PDF Downloads 30 30 4