PLANT REGENERATION OF AVOCADO (PERSEA AMERICANA MILL) IN VITRO

in HortScience
View More View Less
  • 1 Univ of Fla, Tropical Research and Educational Center, 18905 SW 280 St, Homestead, FL 33030
  • | 2 USDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Rd, Miami, FL 33158
  • | 3 Univ of Fla, Tropical Research and Educational Center, 18905 SW 280 St, Homestead, FL 33030

A procedure was developed to regenerate plants via tissue culture from embryonic axes of mature avocado seeds. Explants were cultured in Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and naphthalene-acetic acid (NAA) or thidiazuron (TDZ) and NAA. Culture were kept in the dark for 7-10 days to reduce browning resulting from phenolic oxidation. Multiple shoots (5-8) were formed after transfer to light. Further multiplication were achieved using different combination of BA and NAA or TDZ and NAA. Shoots were cultured in MS supplemented with 2mg/l indolebutyric acid (IBA) for 2 weeks then transferred to MS supplemented with lg/l activated charcoal for root induction. Complete plants were obtained in vitro.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 144 24 2
PDF Downloads 121 50 4