MONOTERPENOID CONSTITUENTS OF ROSEMARY SHOOT TIPS CULTURED IN VITRO

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  • 1 Horticulture Department, and Food Science & Technology Department, University of Nebraska, Lincoln, NE 68583

Shoot tips (7 to 10 mm long) of rosemary plant (Rosmarinus officinalis L. 'Lockwood de forest') were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) (0, 0.5, 1.0, 1.5, and 2 mg/l) alone or with 3-indole acetic acid (IAA) at 0.5 mg/l. The effect of TDZ and IAA on the proliferation of rosemary shoot tips has been reported in a previous meeting. Here, we report on the effect of TDZ and IAA on the monoterpene constituents identified in the oil of rosemary plants propagated in vitro. The proliferated explants were soaked in hexane as a solvent, then the extractions were used for monoterpene analysis using GC/MS. A significant interaction of TDZ by IAA was found on most of the oil components identified. The highest levels of 1,8-cineole and borneol were obtained at 0.5 mg TDZ/l alone, while the highest level of camphor was obtained at 0.5 mg TDZ/l plus 0.5 mg IAA/l. The highest level of bornyl acetate was at 2 mg TDZ/l.

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