RAPD MARKER DIVERSITY AMONG PAKISTANI APRICOTS

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  • 1 Dept. of Pomology, University of California, Davis, CA 95616-8683

A number of 10 base primers were screened to identify RAPD polymorphisms among a population of semi wild apricot genotypes that had been collected by Maxine Thompson in 1988. 30 families collected from trees at 6 locations were analyzed. DNA from leaf tissue of 180 plants, ca. 6 genotypes per family, were isolated and tested against 20 primers. Seven primers were identified that produced consistent results with relatively few (thus, scoreable) and consistent bands. DNA was isolated using the cTAB method and the effects of additional CsCl centrifugation isolation were tested. No differences were found. Reaction conditions were tested to ensure consistent results. Considerable RAPD polymorphism was observed in this population. Parsimony analysis is being conducted to assess the relative variation among and within populations and to determine whether collection location had a more significant effect on DNA variation than other factors such as outcrossing or level of heterogeneity within populations.

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