CALOGENESIS AND ORGANOGENESIS OF SOMATIC MATERIAL FROM APPLE SEEDLINGS (MALUS DOMESTICA BORKH) AS AFFECTED BY SOURCE MATERIAL AND DARK PERIOD.

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  • 1 (EMBRAPA-CNPFT, Caixa Postal 403, 96-100 - Pelotas, RS - Brasil).
  • | 2 (UFV-DFT, Vicosa, MG - Brasil)

The aim of this work was to study different apple of somatic material as callus and adventitious shoots are concerned, for further utilization in the research of somaclonal variation. The somatic materials were: leaf discs, cotyledons and hypocotyls of Gala apple seedlings, cultivated in a MS medium added by B5 vitamins in addition to (in mg/l): BAP (1,0), NAA (0,5) mio-inositol (100,0) sucrose (30,0 g/l) and solidified in agar (6,0 g/l). The several times of explant exposition to the dark affected the final callus weight. Callus weight derived from leaf discs were higher than those for cotyledons and hypocotyls. Explants exposed directly under light or up to two weeks in the dark showed less percentage of regenerative callus as compared to those of three weeks in the dark. The leaf explants presented the highest percentage of regenerative callus. The least response was obtained for those derived from hypocotyls. The highest number of adventitious shoots was obtained keeping the explants three weeks in the dark as compared to directed light exposition.

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