EFFECTS OF PULSED EXCIMER LASER RADIATION ON SHOOT REGENERATION AND REDUCTION OF CONTAMINATION OF PHOENIX DACTYLIFERA L. (DATE PALM)

in HortScience
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  • 1 University of Baghdad, College of Agriculture, Department of Horticulture, Abu-Grahib, Baghdad, Iraq

The seeds of two cultivars of Phoenix dactylifera L. (Medjol and Deglet-Nour) were cultured on modified Murashige and Skoog (MS) medium containing 0.5 mg/l NAA and 2.0 mg/l BA. Later they were treated with 25 or 50 pulses of excimer laser radiation. The results indicate that these seeds exhibited significantly less contamination than control. The highest percentage germination for both cultivars was obtained with explants treated with 50 pulses excimer laser radiation. Compared to other treatments, the occurrence of somatic embryo-genesis and shoot regeneration was greater with the Medjol cultivar.

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