Meristem Micropropagation Protocols for Vitis rotundifolia Michx.

in HortScience
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  • 1 Department of Plant Pathology and Weed Science, Drawer PG, Mississippi State University, Mississippi State, MS 39762

A meristem micropropagation system was developed to produce Agrobacterium -free muscadine grape. Meristems were cultured on a modified Woody Plant Medium (mWPM) supplemented with 0.45 μm BAP. After 2 weeks, cultures were transferred to mWPM containing 8.92 μm BAP to enhance shoot proliferation. Propagules were subsequently subdivided and transferred to fresh medium at 2- to 4-week intervals. New shoots were excised and inserted in mWPM supplemented with 0.57 μm IAA to promote root formation. This method has been successfully used to produce Agrobacterium -free plants of muscadine cultivars Carlos, Doreen, Jumbo, Magnolia, and Sterling for research purposes and for a foundation planting in Mississippi. Chemical names used: benzylaminopurine (BAP); indole3-acetic acid (IAA).

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