RFLPs Obtained from an rDNA Probe and Detected with Enhanced Chemiluminescence in Apples

in HortScience
Authors: Hilde Nybom1, Susan Gardiner2, and Charles J. Simon3
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  • 1 Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, S-29194 Kristianstad, Sweden
  • | 2 Department of Scientific and Industrial Research-Fruit and Trees, Palmerston North, New Zealand
  • | 3 U.S. Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164
DOI:
https://doi.org/10.21273/HORTSCI.27.4.355
Page Count:
2
Volume/Issue:
Volume 27: Issue 4
Article Category:
Research Article
Online Publication Date:
Apr 1992
Free access

Individual-specific DNA fragment patterns were obtained by hybridization of endonuclease-digested apple (Malus ×domestica Borkh.) DNA with a probe (pAR72) derived from the rDNA spacer region of the `White Angel' crab apple. Fragment detection was carried out with a nonradioactive method, using a horseradish peroxidase-catalyzed luminol oxidation. Paternity could be inferred by comparison of the fragment pattern generated by a seedling with those derived from putative parents.

Volume 27 (1992): Issue 4 (Apr 1992)

in HortScience
Cover HortScience
Copyright:
© American Society for Horticultural Science 1992
Page Count:
2
Article Category:
Research Article
Received:
10 Jul 1991
Accepted:
02 Dec 1991
Print Publication Date:
01 Apr 1992
Online Publication Date:
Apr 1992
Keywords:
Malus domestics ; paternity analysis; DNA fingerprint; cultivar identification; nonradioactive
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