RFLPs Obtained from an rDNA Probe and Detected with Enhanced Chemiluminescence in Apples

in HortScience
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  • 1 Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, S-29194 Kristianstad, Sweden
  • | 2 Department of Scientific and Industrial Research-Fruit and Trees, Palmerston North, New Zealand
  • | 3 U.S. Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164

Individual-specific DNA fragment patterns were obtained by hybridization of endonuclease-digested apple (Malus ×domestica Borkh.) DNA with a probe (pAR72) derived from the rDNA spacer region of the `White Angel' crab apple. Fragment detection was carried out with a nonradioactive method, using a horseradish peroxidase-catalyzed luminol oxidation. Paternity could be inferred by comparison of the fragment pattern generated by a seedling with those derived from putative parents.

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