Leyland cypress [×Cupressocyparis leylandii (A.B. Jacks. and Dallim.) Dallim. and A.B. Jacks.] plants were transplanted into the field monthly from Aug. 1989 through Mar. 1990, and laboratory cold-hardiness estimates of these transplants were obtained monthly for two winter seasons. Cold hardiness estimates obtained in Dec. 1989 and Jan. 1990 revealed that the Nov. and Dec. 1989 transplants were 6C less cold-hardy than those transplanted into the field earlier in the year. There was little difference in cold hardiness due to transplant date during Feb., Mar., and Apr. 1990. In the second year of the study, on the same transplants, cold hardiness varied among transplanting dates. In Dec. 1990 and Jan. 1991, those transplanted in Jan.-Mar. 1990 were up to 9C less cold-hardy than those transplanted earlier in the season. However, in Mar. and Apr. 1991, those transplanted in Jan.-Mar. 1990 were equally or more cold-hardy than those transplanted earlier in the season. Transplanting Leyland cypress into the field in August to November appears to be the best time to ensure development of cold hardiness in early winter, whereas January to March planting appears to promote greater cold hardiness in the spring months.