Five isozyme systems were used to detect zygotic seedlings from five polyembryonic cultivars of mango (Manifera indica L.). Significant differences were found between cultivars (x2 = 35.53, P < 0.001) for the percentage of zygotic and nucellar seedlings detected. The range of variation in the percentage of off-types was from 0% in 13-1 to 64% in Golek. The percentage in Sabre was 4%, and 24% and 36% in Tupentine and Madoe, respectively. Three of eight rootstock mother trees of Turpentine were determined to be off-types.