Propagation of Sterile Ornamental Pepper by Cuttings and in Vitro Shoot-tip Culture

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  • 1 Department of Horticulture, University of Georgia, Coastal Plain Experiment Station, Tifton, GA 31793

A sterile (nonflowering) mutant pepper plant (Capsicum sp.) with large, purplish-green, leathery leaves and purplish-green stems was observed during a hybridization program for ornamental peppers. Propagation of this mutant was investigated using cuttings rooted in the greenhouse and in vitro cultures. The most suitable treatment for rooting of cuttings involved the use of two-node cuttings, 2 to 4 mm in diameter, treated with Rootone and rooted in Promix inside a humidity chamber kept in 60% shade. With this treatment, 40% of the cuttings had rooted after 8 weeks. Two-node shoot tips callused when cultured in vitro in Murashige and Skoog (MS) medium containing 4.9 or 9.8 μm IBA or 8.8 μm BA, but 60% had rooted after 8 weeks in half-strength MS medium without growth regulators. Chemical names used: 1 H -indole butyric acid (IBA), N -(phenylmethyl)-1 H -purin-6-amine (BA).

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