Natural-light growth chambers constructed within a greenhouse compartment were equipped with a ventilation/circulation system, two stages of heating, and evaporative cooling. Air drawn from the greenhouse compartment continuously ventilated the chambers; the air was heated or cooled to the set-point temperature. A computer-controlled environmental system maintained uniform temperatures within the chambers and maintained the temperature within ±1C of the set point at night and during periods of low solar radiation; during higher solar radiation periods, control was not as precise. Carbon dioxide concentration was accurately maintained, and the photosynthetic photon flux from supplemental high-pressure sodium lamps was ≈200 μmol·m-2·s-1. The natural-light growth chambers provide a means for studying the interactive effects of temperature, light, and other environmental variables in experiments to increase production efficiency.