Germination and Regeneration of Plants from Old Cucumber Seed

in HortScience

Cucumber (Cucumis sativus L.) seeds of `Marketer', `Marketmore', `Wisconsin SMR-18', `Tablegreen', `Spotfree', and `China' were stored at 3C and 38% relative humidity for up to 26 years. Seed older than 13 years did not germinate. Cultivars stored 10 years gave 80% germination, except Wisconsin SMR-18' (40%). Ten-year-old seeds were separated from their seedcoats, and cotyledons were excised into six segments. Explants were placed on Murashige and Skoog medium with all combinations of BAP (0, 1,2, and 3 mg·liter-1) and NAA (0, 0.1,0.2, and 0.3 mg·liter-1). Plants were obtained from culture for all cultivars grown on medium containing NAA and 1 mg BAP/liter. No plants were regenerated when BAP or NAA was lacking. Chemical names used: benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA).

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