Plant Regeneration from Cotyledons of Cucumis melo `Topmark'

in HortScience
Author: Paula P. Chee1
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  • 1 Molecular Biology Research, The Upjohn Company, 301 Henrietta Street, Kalamazoo, MI 49007

A procedure for the regeneration of muskmelon (Cucumis melo L.) cv. Topmark via shoot organogenesis from cotyledon explants is described. The best induction medium for a morphogenic response was MS salts and vitamins medium with BA at 1.0 mg·liter-1. Further vegetative bud development was completed by transferring organogenic tissue to MS medium containing BA at 0.05 mg·liter-1. The shoots were rooted in MS medium containing NAA at 0.01 mg·liter-1. Morphologically normal plantlets were obtained. Chemical abbreviations used: 6-benzylaminopurine (BA); indoleacetic acid (IAA); naphthaleneacetic acid (NAA).

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