Green pods of the C. reginae orchid were collected from a bog near Ithaca, NY. Pods were surface sterilized, and seeds were plated on agar media. The 8 germination treatments were arrange in a complete 3 way factorial consisting of 2 basal media (fish emulsion, FE vs. yeast extract, YE), 2 media pHs (4.8, 6.8), and 2 temperature regimes (constant 24 C vs. 6 wks. at 5 C prior to transfer to 24 C). Sequential stages of development included embryo enlargement and rupture of the testa (l), root elongation (2), leaf primordium development (3) and finally rhizoid development with or without protocorm greening (4). After 4 months post sowing, germination (stage 1 or beyond) was 56% and 0% on FE at pH 4.8 and 6.8 respectively, and 45% and 78% on YE at pH 4.8 and 6.8 respectively. Protocorm development from unchilled seeds after 4 months was greatest on YE at the lower pH, with 14% reaching stage 3 or 4, as contrasted to only 5% reaching stage 2 (none beyond), for the other germinated treatments. Chilled seeds had higher germination for all treatments but no development beyond stage 1 at 4 months post sowing.
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