Pear in Vitro Propagation Using a Double-phase Culture System

in HortScience
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  • 1 Laboratorio de Fisiología Vegetal, Facultad de Biología, Universidad de Oviedo, Oviedo, Spain
  • | 2 Ente Nationale Delle Energie Alternative, Centro Ricerche Energia Cassacia, Rome, Italy

Proliferation of Pyrus communis L. cv. Abate Fetel, Precoce Morettini, and Guyot was accomplished with a yield of 10 to 15 new shoots per explant. The in vitro procedure is based on the use of 6.7 μm BAP as an overlay on a modified MS medium. Rooting without callus formation was achieved by immersing the basal end in 5 μm IBA solution for 1 min. The possible inhibition of proliferation and plantlet regeneration by GA3 and IBA is discussed. Chemical names used: 6-benzylaminopurine (BAP); indole-3-butyric acid (IBA); gibberellic acid (GA3).

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