Genetic recombination rates of hybrid plants regenerated from three tissue culture. systems were compared by backcrossing regenerated plants with mutant parents and comparing the observed crossover frequencies with those expected based on control plants raised from seed. Increased recombination rates and map distances were observed among plants from micropropagated shoot tips (4.5%-5.9%), cotyledon calli (3.7%-8.5%), and thin cell layers (2.8%-6.5%) between the sunny (sy) and baby leaf syndrome (bls) markers which flank the centromere on chromosome 3. Conversely, a decrease in map distance was observed between bls and the solanifolia (sf) locus which is more distal to the centromere on the same arm of chromosome 3 as bls. Increased map distance among plants regenerated from micropropagated shoot tips, cotyledon calli, and thin cell layers was also observed between white virescence (wv) and anthocyanin reduced (are) loci on chromosome 2.