EVALUATION OF SOMACLONAL VARIATION FOR RESISTANCE TO MYROTHECIUM RORIDUM IN REGENERANTS OF CUCUMIS MELO L. FROM COTYLEDONARY CALLUS

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  • 1 Department of Horticulture, University of Maryland, College Park, MD 20742 USA
  • | 2 Plant Molecular Biology Laboratory, ARS, USDA, Beltsville, MD 20705

Cucumis melo L. (muskmelon) is susceptible to Myrothecium roridum at all stages of growth with no known source of resistance. Screening of regenerants from long-term cotyledonary-derived callus cultures of muskmelon cv. Hales Best (tolerant), Iroquois (susceptible), and Perlita (intermediate) was carried out to determine if novel plants with increased levels of resistance could be obtained. A detached-leaf bioassay was used to screen greenhouse-grown regenerants and seedlings of the three cultivars. Resistance was determined by measuring necrotic lesion diameter and chlorotic plus necrotic lesion diameter. No change in the level of resistance to M. roridum has thus far been observed. Thus, screening for somaclonal variation may not be aviable approach to recover resistance in muskmelon to M. roridum.

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