APPLICATION OF CALLUS CULTURE FOR THE STUDY OF BLACKSPOT DISEASE RESISTANCE IN ROSES

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  • 1 Departments of Horticulture and Plant Pathology, Mississippi State University., Miss. State, MS 39762.

The objective of this study was to determine whether tissue culture can be used for studying the blackspot resistance found in some roses. Callus was initiated from leaves, petioles, and stems of resistant and susceptible genotypes. Good callus formation for susceptible roses (hybrid teas) was obtained on a medium containing MS basal salts, vitamins, sucrose, and 8 g/L agar supplemented with 2 mg/L 2,4-D, 1 mg/L NAA, and 0.2 mg/L BA. Callus formation for resistant roses (species roses) was best when the concentrate ions of growth regulators in the medium were halved. Browning in species rose cultures, was decreased with the addition of 0.05 g/L ascorbic acid to the medium followed by incubation in the dark. The subculture calli were inoculated with the fungal conidia and were analyzed for proteins by SDS-PAGE. These protein profiles were compared to those of whole leaf samples. The results are discussed in terms of similarities and differences in the biochemical responses of callus cultures versus whole leaves to the blackspot infection.

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