IN VITRO REGENERATION FROM CALLUS OF ROSMARINUS OFFICINALIS

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  • 1 Department of Horticulture, University of Nebraska, Lincoln, NE 68583-0724

Regeneration from callus of rosemary has not been reported. Leaf segment, meristem-tip and shoot-tip explants of Rosmarinus officinalis were cultured on a Murashige and Skoog (MS) medium supplemented with five concentrations of the cytokinin thidiazuron (TDZ) alone or in combination with 3-indoleacetic acid (IAA). Callus was formed on the base and leaves of the shoottips after 6 weeks when cultured under cool white fluorescent light (26 u mol·S-1 m-2) on MS containing 0.5, 1.0, 1.5 or 2.0 mg/l TDZ. Calti were transferred to fresh MS medium supplemented with 0.2, 0.4, 0.6, 0.8 or 1.0 mg/l TDZ or 2.0, 4.0, 6.0 or 8.0 mg/l benzyladenine (BA) where shoot formation occurred. Essentiality of IAA was not clear from these experiments and further research is underway to refine regeneration protocol

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