LINKAGE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS AND ISOZYMES IN A BACKCROSS OF CITRUS AND PONCIRUS

in HortScience

Genetic linkage analysis was performed on an interspecific backcross of citrus [Citrus grandis (L.) Osbeck cv. Thong Dee X (Thong Dee X Poncirus trifoliata (L.) Raf. cv. Pomeroy)], using restriction fragment length polymorphism (RFLP) and isozyme analysis. Sixty-five progeny were analyzed for a total of 57 segregating markers including 9 isozymes and 48 RFLPs. Significant (p = 0.05) deviation from an expected 1:1 segregation ratio was observed for 21 (37%) of the 57 loci, but this did not exclude their use in the mapping study. Linkage analysis revealed that 50 loci mapped to 12 linkage groups while 7 loci segregated independently from all other markers. The total map distance included in the 12 linkage groups was 472 cM with the mean distance between markers being 12.8 cM. This does not represent a saturation of the genome with markers; however, this work demonstrates the potential for mapping traits of economic importance in citrus.

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