EFFECT OF SALT SHOCK ON PHOTOMIXOTROPHIC SUSPENSION CULTURES OF SOYBEAN

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  • 1 Department of Horticultural Sciences, College Station, TX 77843

When plants are subjected to stress conditions, they are believed to be developing defensive mechanisms. Those mechanisms could be studied by analysing and comparing the proteins from stressed and nonstressed plant materials. Photomixotrophically grown soybean suspension cultures were shocked with 150 mM, 200 mM, and 250 mM salt concentrations for 1 hr. and 3 hrs. The cells were then given 2, or 4 hr. recovery period. After treatment, proteins were quantified, using Bradford Assay, and then separated on SDS PAGE gels. In Coomasie stained gells, there were different banding patterns in shock treated samples, compared to the control. But there were no differences identified between different shocking times or recovery period treatments. The results from Silver staining and growth studies will be presented.

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