TISSUE CULTURE OF CUPHEA GLUTINOSA CHAM. & SCHLECHT.

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  • 1 Dept. of Horticulture, Coastal Plain Expt. Station, Univ. of Georgia, Tifton, GA 31793
  • 2 USDA/ARS, Coastal Plain Expt. Station, Tifton, GA 31793

Caphea glutinosa is a herbaceous, low-growing annual, bearing numerous attractive purple flowers and has potential as an ornamental and as a ground cover. Plants exhibit winter hardiness in USDA plant hardiness zone 8. Tissue culture techniques were developed to obtain large numbers of uniform plants. Whole leaf explants (approximately 1.0 cm2) callused profusely in MS (Murashige and Skoog, 1962) medium containing 84 mM sucrose, 1% (w/v) Difco Bacto agar and 8.8 μM N6benzyladenine. Shoot formation from calli was observed in the same medium 4 weeks after explanting. Detached shoots were rooted (100%) in half strength MS medium and rooted shoots were transferred to Promix® in the greenhouse 2 weeks after rooting. Tissue cultured plants flowered after 60 days in the greenhouse and no phenotypic differences were observed in floral or foliar characteristics.

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