EFFECT OF AUXIN SOURCE AND EXPLANT TYPE ON MORPHOGENESIS IN VITRO OF THREE TOMATO INBRED LINES

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  • 1 Department of Horticulture, Clemson University, Clemson, SC 29634

Cotyledon explants and zygotic embryos of Lycopersicon esculentum H132, OH8442, and OH2253 were cultured on Murashige and Skoog medium containing varying concentrations of 2,4-D and NAA with and without 10-7 M zeatin. NAA above 10-5 M and 2,4-D above 10-6 M inhibited root formation from cotyledons. Zygotic embryos were removed from developing ovules at the globular, heart, and torpedo stages and later germinated on hormone-free medium. Globular structures that resembled immature zygotic embryos were produced at NAA concentrations between 10-4 and 10-3 M and 2,4-D concentrations between 10-5 and 10-4 M. Treatments reported to enhance maturation and germination of somatic embryos of other species, including subculture to a hormone-free medium with and without activated charcoal, the addition of an ABA treatment subsequent to the auxin treatment, isolation of individual structures from the explant, and a liquid medium rinse containing activated charcoal, have not been successful in stimulating further development of the globular structures.

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