EVALUATION OF STRAWBERRY (FRAGARIA SP.) SEEDLINGS FOR DROUGHT AND SALT TOLERANCE BY IN VITRO INDUCED STRESSES.

in HortScience
Authors:
Gayle VolkDept. of Horticulture, Colorado State Univ., Fort Collins, CO 80523.

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Virgil EsenseeDept. of Horticulture, Colorado State Univ., Fort Collins, CO 80523.

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Harrison HughesDept. of Horticulture, Colorado State Univ., Fort Collins, CO 80523.

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Crosses and self's were made among Fragaria × ananassa Duchn. cv. `Douglas' and `Fern' and Fragaria chiloensis (L.) Duchn. Seeds were surface sterilized, germinated and then grown on MS media (no vitamins, sucrose or hormones) with NaCl concentrations of 0 to 0.5% or 0.5% KCl. Polyethylene glycol (PEG), of corresponding water potentials, was used to induce drought stresses. Whole plant dry weights were evaluated after 50 days. Differences in salt tolerance were associated with genotype; progeny involving crosses with F. chiloensis showed greater salt tolerance. Increases in concentration of PEG caused decreased growth. The use of salt containing media may be used to evaluate strawberry seedlings for salt tolerance and, similarly, PEG may be used to evaluate for drought stress in vitro.

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